10^th World Congress on Veterinary & Animal Science May 18-19, 2018 Osaka, Japan

Min-ji Kim has completed her bachelor’s degree at the age of 23 years from Kangwon National University in Korea. And now she is in master’s course and study about ruminant nutrition at College of Animal Life Science, Kangwon National University

This study was conducted to investigate the effects of nutrient intake level on blood metabolism related to reproduction efficiency in Korean native Hanwoo cows. Thirty cows were assigned to one of two dietary groups; CON (concentrates 3.6 kg and rice straw 3 kg) and TMR (TMR 6.6 kg). The dry matter intake higher in CON (6.05 kg) than in TMR (5.83 kg). While the protein and TDN intake were higher in TMR than in CON. The blood NEFA concentration of TMR and CON was 219.93 uEq/L and 119.53 uEq/L, respectively and blood glucose concentration of CON was increased by 36% compared to TMR. Meanwhile, the BUN was significantly higher (p < 0.0001) in TMR (20.45 mg/dL) than in CON (14.49 mg/dL). The blood concentration of AST and GGT was higher in CON than in TMR. The results of the above study showed that when the TMR was fed, the NEFA concentration was significantly increased and the glucose and BUN were decreased than those of concentrate feeding. These differences of the blood metabolites concentration was caused in the levels of nutrient intake. Since the blood metabolites are known to be related to reproduction efficiency, therefore, the feed type and nutrient intake level that can affect blood metabolites are important for productivity in Hanwoo cows.

Shiela Francisco Margallo is a Veterinarian at Biological Resource Centre- Agency for Science, Technology and Research (A*STAR), Singapore. She obtained her veterinary degree from the University of the Philippines, Los Banos, Laguna. She is a veterinary professional with 10 years of combined technical experience in Sales & Marketing (Animal Health Division), Companion Animal Practice and Biomedical Research. She is experienced in handling rodents, rabbits, swine and NHPs. Among her current roles are to provide laboratory animal medical and surgical care by examination, treatment and advice to ensure adequate and humane veterinary support; safeguard, monitor and advise on the welfare of animals; advise on and assist implementation of the principles of replacement, reduction and refinement; ensure that company policies and practices regarding animal care and welfare are in compliance with relevant regulations. She sits at the Institutional Animal Care and Use Committee. She completed her LabVet Europe programme from University of Copenhagen, Denmark in 2017

Complete Blood Count (CBC) is an essential tool in evaluating overall animal health and detecting diseases. The stability of blood samples in various storage and temperature conditions to determine if they provide consistent results is well- studied in humans. In laboratory animals, studies in rats, mice and monkeys were conducted. However, limited studies exist on rabbits. The aim of this study is to find out if prolonged periods of storage and various temperatures influence the stability of CBCs in rabbit whole blood. Blood collection was done in towel-wrapped rabbits with local topical anaesthetic cream applied on the collection site thirty minutes prior to collection. One millilitre of blood was collected from sixteen New Zealand White rabbits in EDTA tubes and distributed into two aliquots - one stored at room temperature (21 degrees) and the other at refrigerator temperature (4 degrees). Baseline value was measured after blood collection and at various time points (6, 24, 48, and 72 hours) using an impedance technology haematology analyser. The stability of CBC parameters was determined by comparing the results of each time point to the baseline reading. The differences were evaluated using One-way Analysis of Variance (ANOVA) repeated measures after verification of normal distribution by ChiSquare test. Friedman test was used for parameters with non-normal distribution patterns.The studied parameters include White Blood Cell Count (WBC), Red Blood Cell Count (RBC), Haemoglobin Concentration (HGB), Haematocrit (HCT), Platelet Count (PLT), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), RBC Distribution width (RDW) and Mean Platelet Volume (MPV). Results showed that most parameters were stable at 4 degrees up to 24 hrs except for Hgb (p=0.0079) and MPV (p=0.0015) which values started to increase from 6 hours. At room temperature, most values were unstable starting from 6 hours - RBC (p<0.0001), Hgb (p<0.0001), MPV (p=0.0015), HCT (p=0.0475), PLT (p=0.0146). It was concluded that rabbit blood samples can be suitable for haematological analysis up to 24 hrs at 4 degrees storage. However, it is still advisable to process the blood samples immediately after collection to yield better and reliable results

Tzu Chao has completed my university degree at the age of 22 years from Tunghai University and major in the Department of Animal Science and Biotechnology. Then, I have an advanced study for my master degree in Animal Biotechnology now and interested in genome of livestock. However, I have never been to other countries to visit the livestock field or join the conference before. So, I hope to learn more knowledges or get more academic exchanges by participating the conference, in order to broaden my horizons. Furthermore, I will improve and revise my studies based on the recommendation from related researchers and the discussion with my dissertation committee

The velvet antler is an organ that has important biological significance in deer, and its growth is a complicated biological metabolism process. The growth of antlers is correlated with the seasonal variation of serum androgens. Moreover, seasonal levels of testosterone in plasma are well established, steroid concentration have been determined in the tissues of growing antlers. However, the molecular mechanism underlying the process remains elusive. Previous research suggests that genetics factors play essential roles in the weight of velvet antlers. In this study, we screen weight related genes of velvet antlers by Gene Ontology (GO) and pathway analysis of whole genome in Formosan Sambar Deer (Rusa unicolor swinhoei). The investigation and sampling is conducted in five deer fields, blood and antler tissue of 7-year-old Formosan Sambar Deer (Rusa unicolor swinhoei) were collected from March to May. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identifies the major signaling pathways involved. The results showed that 10, 7, 13, and 8 genes has been respectively screened in steroid biosynthesis, steroid hormone biosynthesis, terpenoid backbone biosynthesis, and primary bile acid biosynthesis pathways. However, there is no gene screened in steroid degradation and brassinosteroid biosynthesis pathways. Collectively, we screened genes related to the velvet antler weight in Formosan Sambar Deer by Gene Ontology (GO) and pathway analysis of whole genome and identified 38 genes related to the velvet antler weight. We hope that the results will contribute to further mechanistic studies of velvet antler development and compare the diffirences in every age-group of deers