Biography:

Bernard Turek has completed his PhD in 2000 year from Warsaw University of Life Sciences, Poland. He is the head of Surgery Division in Large Animals Diseases Department with the clinic Warsaw University of Life Sciences. He is mainly focused on the treatment of fractures in horses and fixations technics. The results of his clinical work were presented at many international congresses and published in veterinary literature.

Abstract:

Diaphyseal fractures of the III metacarpal bone represent 22% of all fractures of the long bones in horses (McClure). Treatment of such cases is difficult. The most popular solution used in these types of fractures is two plates applied directly to the bone surface, but they are not applicable on contaminated and infected fractures. External fixators are quite commonly used in human medicine, although in veterinary practice there is no typical stabilizer designed for the treatment of diaphyseal fractures of the III metacarpal bone so far. In this study, an external semicircular fixator of our own design was used and in vitro strength tests were conducted to determine the maximum force which would lead to the destruction of non-fractured bone and fractured bone treated with the stabilizer. On the basis of the strength tests, we can conclude that the stabilizer can be strong enough to allow the horse to stand up after surgery. It also has many favorable features which make it easy to assemble and to take care of a wound, while being safe enough for the animal at the same time.

Biography:

Chaohui Dai is a doctoral student of Yangzhou University, her manger is Animal Genetics, Breeding and Reproduction. And she is mainly engaged in pig disease-resistant breeding and reproduction.
 

Abstract:

Toll-like receptor 5 (TLR5) plays an important role in the immune system. In this study, we performed transcriptome sequencing of the duodenum in E. coli F18-resistant and -sensitive Sutai weaned piglets and analyzed the differential expression of TLR5. The cellular localization of TLR5 was investigated, the small intestinal epithelial cell line (IPEC-J2) with TLR5 gene overexpression and interference was established. Following stimulation of IPEC-J2 cells by E. coli, we evaluated the effect of TLR5 expression on E. coli invasion. The results showed that TLR5 expression level in the duodenum and jejunum was significantly higher in sensitive than in resistant piglets. IPEC-J2 cell lines with TLR5 interference and overexpression were established, and the expression of genes associated with the TLR5 signaling pathway were significantly higher in TLR5-overexpressed cells than in control cells. Bacterial adhesion was higher in TLR5-overexpressed cells than in blank cells and lower in TLR5 interference than in blank cells. The core promoter region of TLR5 included two CpG islands and 15 acting elements. The methylation of the mC-6 site in the second CpG island of the promoter region had a regulatory effect on TLR5 expression. Therefore, low expression of TLR5 inhibited the immune response to some extent and decreased cell damage, which was conducive to the resistance to E. coli infection. The methylation of the mC-6 CG site in the second CpG island of the TLR5 promoter inhibited the binding of the transcription factor Sp1, which decreased TLR5 expression and affected E. coli resistance.

Biography:

Maria Chrenkova is a Scientist with 38 years of experience in ruminant nutrition and feeds evaluation. Her research works are focused on feed quality, parameters of ruminal degradability, intestinal digestibility and biological value of proteins using model animals (rats and rabbits). She also studies the use of genetically modified and non-traditional feeds in animal nutrition and their effects on animal health and quality of animal products. She is involved in several national and international projects related to feed quality, animal nutrition, metabolism and production.

Abstract:

GM maize with acquired resistance to the European corn borer and other Lepidopteran pests - MON 810; GM maize with resistance to Lepidopteran and corn rootworm larvae and with tolerance to glyphosate - MON 88017 x MON 89034; GM maize with resistance to Lepidoptera and tolerance to glyphosate - MON 89034 x NK 603 were compared with their isogenic lines. All tested samples of maize were grown in equal climatic and soil aggregate conditions. Nutritional value of GM maize was tested on model animals - rabbits, which were fed 12% share of corn in feed mixture during full terms of fattening. No influence of GM maize on growth, feed conversion, parameters of fermentation process in the cecum of rabbits and on animal health was determined. In meat of rabbits that are handed feed mixtures with contents of GM maize, determined are equivalent values of pH, contents of protein, fat, energetic values, essential amino acid and the fatty acid profile as in meat rabbits, that were served feed mixture with izogenic maize lines. Maize grain is used in nutrition of ruminants for the high content of starch, but also for important contents of crude protein. For GM maize MON 89034 x NK 603, izogenic maize and 3 references hybrids, effective degradability dry matter, crude protein, organic matter and NDF were determined. Obtained results indicate there are no differences between GM and izogenic maize. Acknowledgment: „This work was supported by the Slovak Research and Development Agency under the contract  No.  APVV-15-0477“.

Biography:

Tzu Chao has completed her university degree at the age of 22 years from Tunghai University and major in the Department of Animal Science and Biotechnology. Then, she has an advanced study for my master degree in Animal Biotechnology now and interested in genome of livestock. However, she has never been to other countries to visit the livestock field or join the conference before. So, she hope to learn more knowledges or get more academic exchanges by participating the conference, in order to broaden my horizons. Furthermore, she will improve and revise my studies based on the recommendation from related researchers and the discussion with my dissertation committee.

Abstract:

The velvet antler is an organ that has important biological significance in deer, and its growth is a complicated biological metabolism process. The growth of antlers is correlated with the seasonal variation of serum androgens. Moreover, seasonal levels of testosterone in plasma are well established, steroid concentration have been determined in the tissues of growing antlers. However, the molecular mechanism underlying the process remains elusive. Previous research suggests that genetics factors play essential roles in the weight of velvet antlers. In this study, we screen weight related genes of velvet antlers by Gene Ontology (GO) and pathway analysis of whole genome in Formosan Sambar Deer (Rusa unicolor swinhoei). The investigation and sampling is conducted in five deer fields, blood and antler tissue of 7-year-old Formosan Sambar Deer (Rusa unicolor swinhoei) were collected from March to May. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identifies the major signaling pathways involved. The results showed that 10, 7, 13, and 8 genes has been respectively screened in steroid biosynthesis, steroid hormone biosynthesis, terpenoid backbone biosynthesis, and primary bile acid biosynthesis pathways. However, there is no gene screened in steroid degradation and brassinosteroid biosynthesis pathways. Collectively, we screened genes related to the velvet antler weight in Formosan Sambar Deer by Gene Ontology (GO) and pathway analysis of whole genome and identified 38 genes related to the velvet antler weight. We hope that the results will contribute to further mechanistic studies of velvet antler development and compare the diffirences in every age-group of deers.

Biography:

Weiyun Qin is a doctoral student of Yangzhou University, his manger is Animal Genetics, Breeding and Reproduction. And he is mainly engaged in pig disease-resistant breeding and reproduction.

Abstract:

The intestinal barrier is closely related to intestinal diseases. We selected 43 piglets to screen candidate genes, 16 piglets were removed by detecting serum D-lactate and DAO levels, then the remaining piglets were preliminary identified as the intestinal barrier-integrated and -impaired groups. The integrity of intestinal barrier was further verified by scanning and transmission electron microscope observation combined with paraffin sections and several stainings. The results showed that the intestinal barrier was completed and structured in typical intestinal barrier-integrated piglets, the length and width of villus were significantly higher than impaired individuals, and the outline of epithelial cells was clear and arranged in a regular manner with relatively abundant goblet cells. The intestine of typical impaired piglets appeared obvious lesions, and villi fell away with lamina propria shedding, glandular atrophy, and a few goblet cells. Finally, we obtained 10 integrated individuals and 12 impaired individuals. On this basis, we used real-time PCR to detect the expression differences of 25 candidate genes between the two groups. The results showed that most genes were significantly different between the two groups. Hierarchical clustering and correlation analysis revealed that there were two important candidate genes GLP2 and AQP3 with strong positive correlation. Finally, we successfully constructed the evaluation system of the intestine barrier integrity in piglets, which provided a valuable material for studying the intestinal barrier. In addition, we speculated that GLP-2 and AQP3 may play an important role in maintaining the integrity of intestinal barrier by affecting the expression of tight junction proteins.

Biography:

Xuemei Yin is a doctoral student of Yangzhou University, her manger is Animal Genetics, Breeding and Reproduction. And she has published 4 papers at home and abroad.

Abstract:

Myofactor-6 gene (Myf6) is a member of myogenic regulatory factors (MRFs) family and plays an important role in regulation of muscle building. To explore the expression profile and expression pattern of Myf6 gene and its possible mechanism of action, in this experiment, we use Real-time fluorescent quantitative PCR to detect the expression level of Myf6 in different tissue to draw the expression profile. Then, the expression levels of Myf6 gene in high expression tissue of breast and leg muscle at five time points were detected to study the expression pattern. The results showed that Myf6 mainly expressed in breast and leg muscle and the expression level was extremely significantly higher (P<0.01) than that in spleen tissue. In Jinghai Yellow chicken, the expression of Myf6 gene in male and female chicken is basically the same, the expression level of breast muscle decreased slightly at 4 weeks, but it showed an increasing trend on the whole, the expression level of leg muscle is rising from 0 weeks to 16 weeks and reaching the highest level at 16 weeks; In Jinmao yellow chicken, the expression of breast muscle and leg muscle showed an increasing trend from birth to 4 weeks and decreased slightly at 8 weeks, then showed an upward trend. This study revealed the expression profile and expression pattern with the growth and development of muscle of Jinghai Yellow chicken and Jinmao Yellow chicken, and might lay a foundation for further study on mechanism of action and expression regulation of Myf6 gene.

Biography:

Moharrery has completed his PhD at the age of 35 years from University of Agricultural Sciences and he has found two sabbatical opportunities in Aarhus University from Denmark. He has published 10 books in native language (Persian) and more than 50 papers in reputed journals and he has served as a peer reviewer for a number of academic journals and conferences.

Abstract:

Feeding highly fermentable carbohydrate resulted the huge amount volatile fatty acids (VFA) production especially lactic acid in un-adapted rumen. The role of rumen microbe on conversion of lactic acid to propionate is well documented. On the other hands, numerous studies have been determined the role of butyrate on the growth and development of rumen epithelium. In this manner, present study has been investigated the feeding of different levels of sodium butyrate in transition cows and its effects on lactic acid consumption by rumen fluid in the early of lactation. Twelve Holstein dry cows in the late of pregnancy divided into three groups with one group being fed 30 g (daily) of sodium butyrate mixed with wheat bran (T30) and the second group with 60 g (daily) of sodium butyrate mixed with wheat bran (T60) and the third group left without supplementation as a control (Cont). On days after parturition 10 ml rumen fluid was taken by ruminocentesis, and the ability of rumen fluid for lactic acid consumption was determined. The correlation between lactic acid concentration in rumen fluid and the ability of rumen fluid for lactic acid consumption was analyzed using the Bayesian approach, after correction for fixed effect (Na butyrate treatments). The results showed that no significant difference among treatments for lactic acid consumption. However, the 95% highest posterior density (HPD) interval of correlation between above parameters was found from -0.99 to -0.60. In view of the fact that zero value is out of this range, it can be concluded that the correlation (ó †°ρ=-0.84) is significant.

Biography:

Zuzana Formelova is a scientist in the Institute for Nutrition, of the Research Institute for Animal Production Nitra, National Agricultural and Food Centre. Her work is focussed on the problems of utilization of nutrients, nutrient rumen degradability, intestinal digestibility of nutrients by in vivo, in sacco and in vitro methods. She also studies the use of genetically modified and non-traditional feeds in animal nutrition and their effects on animal health and quality of animal products. She is involved in several national projects related to feed quality, animal nutrition, metabolism and production.

Abstract:

The majority of livestock are fed diets based on wheat, barley and corn. Rye is rarely used in the forage industry, although it is a quality cereal (due to the high nutritional value of its proteins). Rye contains higher levels of anti-nutritional factors and soluble non-starch polysaccharides compared to wheat. Adding a suitable enzyme to the diet (for poultry and pigs) almost always helps reduce the negative effects of pentosans in rye. The objective of the study was to determine the nutritional value of rye. Their nutrient characteristics, including dry matter, crude protein, crude fat, ash and gross energy, starch content and composition, and concentrations of non-starch polysaccharides, protein fractions and amino acids, were determined. Dry cows with large rumen and duodenal cannulas were used for determination of nutrient degradability (in sacco method) and for intestinal digestibility (mobile bag method). In the samples of rye, we determined: the average content of crude protein 122 g.kg^-1 DM, crude fibre 24.8 g.kg^-1 DM, average content of albumins + globulins 39%, prolamins 24%, glutelins 17.7%, lysine 0.33 g.kg^-1 DM. We determined in rye 156 g.kg^-1 DM total non-starch polysaccharides, total pentosans 85.5 g.kg^-1 DM. Effective degradability of organic matter was 80.2% and crude protein was degraded in rumen to 79.8% on average. Intestinal digestibility of crude protein reached the value 66.6%. Rye represents a cereal with high energy and nutritive value and can be incorporated into feed mixtures. With feeding higher doses, the nutrition-physiological specifics of individual animal species and categories must be kept. Acknowledgment: „This work was supported by the Slovak Research and Development Agency under the contract No. APVV-15-0477“.

Biography:

Lewis Grant, Veterinarian, worked initially in General Practice but then specialised in Veterinary Public Health working as an OVS until 1998, when he was appointed as an area supervisor and auditor by the Meat Hygiene Service (MHS). He moved to MHS headquarters in 2000 helping to deal with the 2001 FMD outbreak, BSE related controls and editing the MHS Operations Manual. He was responsible for hosting EU Inspections to the UK and visits from Third Country delegations. He was awarded the MBE for Services to the Food Standards Agency in 2011 and became President of the VPHA in 2016.

Abstract:

The use of Closed-Circuit Television (CCTV) in slaughterhouses for the purposes of providing additional supervision of animal handling and slaughter practices is much debated in several countries. CCTV is already often used by Food Business Operators (FBOs) to facilitate them in discharging their regulatory responsibilities requiring them to ensure that animals under their care are handled from arrival, unloading, lairaging to slaughter in a humane manner, in accordance with the current Welfare Regulations. Under Council Regulation (EC) 1099/2009 on the protection of animals at the time of killing, FBOs must have trained operatives with Certificates of Competence (COCs) to handle live animals as well as Standard Operating Procedures (SOPs), and CCTV greatly assists the FBO in ensuring Compliance with the Regulation. However, the application of CCTV remains, in many countries, voluntary and variably regulated. In most cases, the Official Veterinarian does not have access to this system nor the resultant footage to assist him/her in carrying out his verification role with respect to the monitoring of animal welfare standards. As a consequence of undercover filming of animal welfare breaches in some slaughterhouses in the UK, legislation for compulsory CCTV with unrestricted access for OVs is being introduced in England from May 2018.

Biography:

Matus Rajsky focuses his research on nutrition, feeding and ecology of animals, especially on farm ruminants and free living ruminants. He is the Head of Institute for Nutrition at Research Institute for Animal Production Nitra, National Agricultural and Food Centre. He is involved in several projects related to feed quality, animal nutrition, physiology of animal nutrition, feeding of animals, proposals of new feeds. He also studies the relation between nutrition and food of free living ruminants (roe deer, red deer, fallow deer, mouflon and moose) and their impact on environment.

Abstract:

The objective of the study was to compare the nutritional value of various heat-treated (by dry heat: 110 °C and 120 °C - 5 min., 10 min., 15 min., and by wet heat: 800 °C (f. Cimbria Dantoaster) and untreated samples of lupine (Lupinus albus). Degradability of nutrients was determinted using in sacco method (Ørskov and McDonald, 1979), and intestinal digestibility of crude protein by mobile bag method (Van Straalen et al., 1993). Dry cows with large rumen and duodenal cannulas were used for determination of degradability and intestinal digestibility. Suitable heat treatment should decrease solubility of crude protein and also its degradability in rumen and increase bypass of crude protein into small intestine. The treatment however caused a significant decrease in the content of crude protein soluble in NaCl (from 157.21 down to 60.11 g.kg^-1), which indicates suitability of this treatment and better utilization of crude protein in small intestine. Lowest effective degradability of crude protein was determined in lupine treated at 800 °C and highest in the lupine treated at 110 ºC.  In the lupine treated at 800 ºC, 42.7% by pass protein was determined. The results indicate that wet treatment utilizing HTST processing (High Temperature Short Time), decreased the effective degradability of crude protein (from 73.58% to 55.14%) and had positive impact on intestinal digestibility of crude protein by 8.46%. Acknowledgment: „This work was supported by the Slovak Research and Development Agency under the contract No. APVV-15-0477“.

Biography:

Shiela Francisco Margallo is a Veterinarian at Biological Resource Centre- Agency for Science, Technology and Research (A*STAR), Singapore. She obtained her veterinary degree from the University of the Philippines, Los Banos, Laguna. She is a veterinary professional with 10 years of combined technical experience in Sales & Marketing (Animal Health Division), Companion Animal Practice and Biomedical Research. She is experienced in handling rodents, rabbits, swine and NHPs. Among her current roles are to provide laboratory animal medical and surgical care by examination, treatment and advice to ensure adequate and humane veterinary support; safeguard, monitor and advise on the welfare of animals; advise on and assist implementation of the principles of replacement, reduction and refinement; ensure that company policies and practices regarding animal care and welfare are in compliance with relevant regulations. She sits at the Institutional Animal Care and Use Committee. She completed her LabVet Europe programme from University of Copenhagen, Denmark in 2017.

Abstract:

Complete Blood Count (CBC) is an essential tool in evaluating overall animal health and detecting diseases. The stability of blood samples in various storage and temperature conditions to determine if they provide consistent results is well- studied in humans. In laboratory animals, studies in rats, mice and monkeys were conducted. However, limited studies exist on rabbits. The aim of this study is to find out if prolonged periods of storage and various temperatures influence the stability of CBCs in rabbit whole blood. Blood collection was done in towel-wrapped rabbits with local topical anaesthetic cream applied on the collection site thirty minutes prior to collection. One millilitre of blood was collected from sixteen New Zealand White rabbits in EDTA tubes and distributed into two aliquots - one stored at room temperature (21 degrees) and the other at refrigerator temperature (4 degrees). Baseline value was measured after blood collection and at various time points (6, 24, 48, and 72 hours) using an impedance technology haematology analyser. The stability of CBC parameters was determined by comparing the results of each time point to the baseline reading. The differences were evaluated using One-way Analysis of Variance (ANOVA) repeated measures after verification of normal distribution by ChiSquare test. Friedman test was used for parameters with non-normal distribution patterns.The studied parameters include White Blood Cell Count (WBC), Red Blood Cell Count (RBC), Haemoglobin Concentration (HGB), Haematocrit (HCT), Platelet Count (PLT), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), RBC Distribution width (RDW) and Mean Platelet Volume (MPV). Results showed that most parameters were stable at 4 degrees up to 24 hrs except for Hgb (p=0.0079) and MPV (p=0.0015) which values started to increase from 6 hours. At room temperature, most values were unstable starting from 6 hours - RBC (p<0.0001), Hgb (p<0.0001), MPV (p=0.0015), HCT (p=0.0475), PLT (p=0.0146). It was concluded that rabbit blood samples can be suitable for haematological analysis up to 24 hrs at 4 degrees storage. However, it is still advisable to process the blood samples immediately after collection to yield better and reliable results.

Biography:

Min-ji Kim has completed her bachelor’s degree at the age of 23 years from Kangwon National University in Korea. And now she is in master’s course and study about ruminant nutrition at College of Animal Life Science, Kangwon National University.

Abstract:

This study was conducted to investigate the effects of nutrient intake level on blood metabolism related to reproduction efficiency in Korean native Hanwoo cows. Thirty cows were assigned to one of two dietary groups; CON (concentrates 3.6 kg and rice straw 3 kg) and TMR (TMR 6.6 kg). The dry matter intake higher in CON (6.05 kg) than in TMR (5.83 kg). While the protein and TDN intake were higher in TMR than in CON. The blood NEFA concentration of TMR and CON was 219.93 uEq/L and 119.53 uEq/L, respectively and blood glucose concentration of CON was increased by 36% compared to TMR. Meanwhile, the BUN was significantly higher (p < 0.0001) in TMR (20.45 mg/dL) than in CON (14.49 mg/dL). The blood concentration of AST and GGT was higher in CON than in TMR. The results of the above study showed that when the TMR was fed, the NEFA concentration was significantly increased and the glucose and BUN were decreased than those of concentrate feeding. These differences of the blood metabolites concentration was caused in the levels of nutrient intake. Since the blood metabolites are known to be related to reproduction efficiency, therefore, the feed type and nutrient intake level that can affect blood metabolites are important for productivity in Hanwoo cows.

Biography:

Moharrery has completed his PhD at the age of 35 years from University of Agricultural Sciences and he has found two sabbatical opportunities in Aarhus University from Denmark. He has published 10 books in native language (Persian) and more than 50 papers in reputed journals and he has served as a peer reviewer for a number of academic journals and conferences.

Abstract:

Twenty pregnant, native fat-tailed ewes aged approximately 36 ± 1.5 months, with body weights (BWs) of 55 ± 6 kg and totally 100 ± 9 days of pregnancy period, were selected for the experiment. Restricted feeding was applied approximately up to 33% of their requirements for all ewes according to NRC (2007). All data from calculated of the total fat mobilization (g/day) and energy efficiency percentage has been used for evaluation of the models. Due to limited size of data set, the validity of variance estimates and normality assumptions as well as confidence interval estimates may be questionable. Hence, bootstrapping technique was used for re-sampling up to 1200 times. Different mathematical functions consisted of exponential, hyperbolic, power, incomplete gamma, and gamma were evaluated with regard to their ability to describe the relationship between fat mobilization and energy efficiency percentage. Based on the goodness of the fit of different criteria and statistical performance, convergence percentage of the three two-parameter functions along with the Gamma function were considerably higher (100%) than the Incomplete gamma (99.08%) functions, suggesting that these functions would be the best models. However, based the t-test carried out for predicted and observed values (n=24000), the Hyperbolic function showed to be superior to other functions (P = 1.000) with 100% convergence and lower parameter. The results of all functions revealed the fact that decreased energy efficiency percentage was the consequence of higher fat mobilization in ewes at the late of pregnancy.

Biography:

Plamen Kirilov is received Ph.D. degree from the University of Toulouse, France in 2007, he followed as Postdoctoral Associate in Skin Center Laboratories and he joined the Faculty of Pharmacy of Toulouse, as a Training Associate Professor in 2011. In the meantime, he was a researcher at the High School of Chemistry in Rennes and Nancy, France. Dr Kirilov has a keen interests and expertise in the fields of semisolid forms, dispersions and rheology of complex systems. Currently he holds the position of an Associate Professor in the University of Lyon where he is researching "Human and veterinary pharmaceutical technology".

Abstract:

Enrofloxacin (1-cyclopropyl-6-fluoro-7-(4-ethyl-1-piperazinyl)-1,4-dihydro-4-oxo-3-quinoline carboxylic acid) was used as the test drug. Enrofloxacin is an antibiotic belonging to the fluoroquinolone family and is commonly administered to snakes. For the transdermal drug vehicle, Pentravan® cream, an oil-in-water emulsion that uses liposomal technology to ensure reproducible transdermal penetration of active ingredients was used. Prior to initiating the experiment, it was important to consider the optimal area of shed snake skin for enrofloxacin application. Previous studies concluded that the back (dorsal) of shed snake skin, which is thicker than shed belly (ventral) skin, is the best option for drug-permeation studies. This formed the basis of our decision to select the back of the shed skin of Python molurus bivittatus in order to study the permeation of enrofloxacin.  Overall, the goal of the study was to develop the transcutaneous route as a simple viable route for the administration of drugs, including antibiotics, such as enrofloxacin, in herpetological medicine. This would facilitate simple effective treatments especially in cases where extended periods of drug therapy are required.  The results showed that transcutaneous passage of enrofloxacin in reptiles, or at least in Python molurus bivittatus, is feasible. This observation will most probably extend to other molecules, including key therapeutics administered to snakes through less straightforward means. A key implication of such a result is that this would facilitate simple effective treatment by snake owners, especially in cases where extended periods of drug treatment are required.

Biography:

Abstract:

High productivity of animals of different breeds capable of high coefficient to transform the nutrients in feed into protein, fat and other organic substances in the organism with low feed cost per unit of output, due to intensive course of metabolic processes in their organs and tissues, and the intense functional activity of all organs and systems. The decrease in total non-specific resistance and immunobiological reactivity as a result of metabolic disorders severely limit the adaptive properties of the organism to resist a biological (microbes, viruses, etc.) and abiotic (xenobiotics) factors, which in recent years intensively accumulate and concentrate in the external environment. For this reason, the animals rom farms increased the incidence of not only infectious but also non-communicable diseases in the result of layering various toxicoses caused by fungi, salts of heavy metals, pesticides and other substances. Therefore, in a multilevel system of regulation of reproductive function in animals and the mechanisms of the development of obstetrical and gynecological pathologies, along with the endocrine system, play an important role in the processes of free radical oxidation and the functional status of the antioxidant defense system and nitric oxide, which is largely dependent on the trace element status of animals. The deficiency of iodine and selenium is one of the reasons for the imbalance in the antioxidant protection system and metabolism, both in terms of hyposelenium biogeochemical provinces, and in the context of a normal geochemical environment, in connection with the increased requirements of the organism due to elevated metabolism. As a consequence, this leads to a predisposition to multiple diseases, infertility and thyroid dysfunction. An important role in antioxidant regulation of animals are not only compounds of iodine and selenium and other trace elements (iron, copper, manganese, cobalt, zinc, molybdenum etc).

Biography:

Alessandro Di Cerbo obtained the bachelor degree in Medical and Pharmaceutical Biotechnologies at University Vita-salute San Raffaele (Milan, Italy) in 2005. In 2007 achieved the Master degree in Medical Biotechnologies at University of Modena (Italy), in 2011 obtained the title of PhD in Nanoscience and Nanotechnology at the same University and in 2016 he got the Postgraduate Diploma in Clinical Biochemistry at University “G. d’Annunzio” of Chieti (Italy). His scientific activities are highly interdisciplinary, ranging from nanotechnology to nanomedicine, microbiology, nutrition and translational medicine. He has published more than 50 papers in reputed journals.    

Abstract:

In the last years many scientific veterinary reports highlighted the pivotal role of nutrition also as a support in animals before surgical, chemotherapy and radiotherapy interventions. We aimed to evaluate the effectiveness of a dietary supplement named Gold Lotion (GL, 4 ml day for 6 months) as adjuvant of current pharmacological therapy in 18 cats affected by different tumors (digestive system lymphoma, carcinoma and cutaneous fibrosarcoma). Metabolic profile and oxidative stress parameters of cats resulted significantly improved after 6 months of GL supplementation (*p < 0.05, **p < 0.01). The combined use of GL and pharmacological therapy not only significantly ameliorated digestive and hepatic functionality of all cats and reduced the overall overload of free radicals but also improved their quality of life (QoL).

Biography:

Mansour Mayahi has completed his DVM, 1983, Shahid Chamran University of Ahwaz, MVSc, 1992, Indian Veterinary Research Institute Izatnagar, U.P., India. and PhD, 1998. Tehran University, Tehran, Iran. He is the dean of Faculty of Veterinary Medicine of Ahvaz. He has published more than 45 papers in reputed journals and published 9 Scientific books and has been serving as an editorial board member of reputed Iranian journal. Dr. Mayahi research mostly  are avian diseases.

Abstract:

The purpose of study was to examine the effects of IBDV on clinical signs, pathogenesis, virus shedding, cellular and humoral response of poults to avian influenza virus (AIV) H9N2.  A total of 100 day-old commercial poults were divided into 4 equal groups. Groups 1 and 2 were infected with 10⁴CID₅₀ of IBDV by intra-bursal route at a day-old; Groups 1 and 3 were each infected with 10⁶EID₅₀ of AIV (H₉N₂) by the oculo-nasal routes on the 30^th day.  Poults of the 4^th Group served as negative control. All groups were vaccinated against   Newcastle disease vaccine (NDV). Clinical signs were observed twice daily while trachea and cloacal swabs from 3 live birds of each group, and tissue samples from lung, pancreas and kidneys from 3 euthanized birds from each group at 3, 7, 11 and 15 days post avian influenza virus H9N2 inoculation, were collected randomly. Avian influenza virus H9N2 in trachea and cloaca swabs and in the tissues, was confirmed by Real-time polymerase chain reaction (PCR). Spleen on mentioned days were collected and cell mediated immunity was assessed by means of lymphocytes proliferation repose to phytohaemagglutinin. Ten blood samples from each group were randomly collected and were measured by HI and Elisa tests. The study showed that the clinical signs of avian influenza virus(AIV) H9N2 were prolonged and more intensive in the IBD infected group and the mean HI titers to avian influenza virus in the IBD infected group were significantly lower compare to the uninfected IBD group from days 14 post infection (P≤0.05). In the IBD infected group, shedding of avian influenza virus from the trachea, on days 3 and 7, and from the cloaca on days  7 and 11, were significantly higher than uninfected IBD group (P≤0.05). The proliferation of avian influenza virus  in the lung on  days 7, 11 and 15 was significantly higher in the IBD infected birds (P≤0.05). It was concluded that IBD changed the pathogenesis of (AIV) H9N2 and influenza virus replication, while shedding in infected birds increased which resulted in prolonged severity and duration of clinical signs.

Biography:

Vasudha Sulakhe is a Post-graduate student of third semester, studying in Maharashtra Animal and Fishery Sciences University, Nagpur. She has published one paper in Indian Research Journal of Extension Education. She has also participated in various Agriculture and Livestock Exhibitions.

Abstract:

ICT is the foundation of economy and a driving force of social changes in the 21st century. The present study explores the use of different ICT tools by livestock farmers in Maharashtra, India. A total of 120 livestock farmers were randomly selected from Pune and Satara districts of Maharashtra, India. The data was collected with the help of structured schedule by personal interview method. Findings revealed that majority of respondents are in the middle age group (58.33%), belonging to rural area (64.16%), with higher secondary level of education (35.83%). It showed that, mobile phone was the most preferred ICT tool (40.00%) followed by T.V (33.33%) and they used internet on their mobile phones for browsing information related to agriculture and animal husbandry practices (66.66%). Social media like Whatsapp (45.09%) and Facebook (37.25%) are being used by the respondents for sharing information in the field of animal husbandry. Most of the respondents were aware about different android based mobile applications (77.50%) but only 40% of the total respondents used this facility to access information related to animal husbandry. Other most widely used mobile applications were KISAN, Agrostar and Agrowon (45.00%). It was also revealed that, majority of the respondents used mobile phones as a communication channel (27.52%). The constraints encountered in accessibility of mobile applications by majority of respondents included digital illiteracy and, lack of mobile friendly and locally relevant digital content in local languages. Effective use of ICTs will help to improve access of information to farmers so as to achieve the broader objective of improving productivity.

Biography:

Muhammad Sohail Sajid  has completed his PhD from University of agriculture Faislabad and Post Doctoral Training from University of Southern Mississippi, Hattiesburg, USA. He is  currently working as Associate professor in Deprtment of parasitology university of Agriculture Faisalabad. He has published more than 35 papers in international reputed journals and more than 30 papers in national reputed journals, 37 abstracts0papers pblished in proceeding of  international conferences  and 62 in  proceeding of national conferences

Abstract:

Rickettsiosis, caused by a Spotted fever group Rickettsiae (SFGR), is considered as an emerging infectious disease from public and veterinary perspective. The present study reports distribution of SFGR in the host (buffalo and cattle) and vector (ticks) population determined through gene specific amplification through PCR targeting outer membrane protein (ompA). Tick and blood samples were collected using standard protocols through convenient sampling from district Faisalabad. Ticks were dissected to extract salivary glands (SG). Blood and tick SG pools were subjected to DNA extraction and amplification of ompA using PCR. Overall prevalence of SFGR was reported as 21.5% and 33.6 % from blood and ticks, respectively. Hyalomma anatolicum was more prevalent tick associated with SFGR as compared to Rhipicephalus sp. Higher prevalence of SFGR was reported in cattle (25%) population as compared to that of buffalo (17.07%). On seasonal basis high SFGR prevalence was recorded during spring season (48.1%, 26.32%, 17.76%) as compare to winter (27.9%, 21.43%, 15.38%) in vector and host (cattle and buffalo respectively) population. Sequencing analysis indicated that rickettsial endo-symbionts were associated with ticks of the study area. These results provided baseline information about the prevalence of SFGR in vector and host population.

Biography:

Wubishet  Zewdie graduated from Addis Ababa University College of Veterinary Medicine and Agriculture in 2008 by Doctor of Veterinary Medicine (DVM) and completed my MSc in Veterinary Microbiology from the same University in July 2017. I am director of Yabello Regional Veterinary Laboratory. I published about 10 papers in different journals and I am associated editor of Annals of Reviews and Research (ARR) journal at juniper publishers’ organization.

Abstract:

The study was conducted to identify the major reproductive health problems and its associated risk factors in indigenous Borena breed cows in Borena zone in Southern Ethiopia between September 2013 and February 2014. Out of the total 409 cows examined, 195 (47.7%) were having at least one of the reproductive problems identified by either questionnaire interview (n=329) or regular follow up (n=80) of individual cows. The major reproductive health problems identified in the present study were mastitis (21.3%; n=87/409), abortion (12.2%; n=50/409), repeat breeder (10.3%; n=42/409), anestrus (10.3%; n=42/409) and retained fetal membrane (RFM; 7.6%; n=31/409). The rate of abortion increased significantly (p=0.001) with the increase in the stage of gestation. Laboratory findings indicated that brucellosis and mastitis had great roles in reproductive health problems of dairy cows in the study area with prevalence rates of 2.91% and 68.41%, respectively. In conclusion, the study revealed that several reproductive health problems such as mastitis, abortion, repeat breeder, anestrus and RFM are mostly prevalent in dairy cows in Borena zone in southern Ethiopia.

Biography:

Abstract:

The second most common neurodegenerative disease is the Parkinson’s disease (PD). Parkinson’s disease is the complex multifactorial neurodegenerative disorder. The symptoms due to the neuro-degeneration may be the stiffness or slowing of movement (bradykinesia), rigid muscles, impaired posture and balance, loss of automatic movements and speech or writing changes. The exact cause for the disease still remains unknown. Based upon the symptoms, series of clinical studies are carried out and it was discovered that there was deficit in basal ganglia dopamine which was involved in the controlling involuntary and voluntary movements. Another development towards understanding the pathology was discovery of lewy bodies in the brain region. Due to the mutations in the sequence of alpha-synuclein protein which is present in the brain cytosol, results in the formation of lewy bodies. In the recent literatures it is reported that aggregation of alpha-synuclein protein could be the reason for the death of dopaminergic neuron cells. There are no available therapies which can alter the underlying neurodegenerative process but symptomatic therapies can help PD patients improve the quality of life. The most important triumph in modern medicine is to understand the pathophysiology and development of novel modes of treatment for Parkinson’s disease. As the disease is multifactorial, it has to be tackled with combinatorial therapy. As there are no reports available on combo therapy for Parkinson’s disease, so theoretically we can claim that 80% of the disease can be cured by using different combination of drugs simultaneously. Behavioural assessments were carried out to investigate the effectiveness of combo drug and methanol extractin MPTP induced PD mice models. Locomotor and Rotarod activity was significantly increased on 14th, 21st, 28th day in MPTP mice models treated with combo-drug and Methanol Extract.

Biography:

Muhammad Sajid has completed his PhD recently in August 2018 from University of Veterinary and Animal Sciences, Lahore, Pakistan. He is working as Assistant Professor and also Incharge of Pathology section at College of Veterinary and Animal Sciences, Jhang, Pakistan. Dr. Sajid is a young teacher and researchers and he has published more than 10 papers in reputed journals.

Abstract:

Heavy metal toxicity is increasing day by day in developing countries like Pakistan. Increasing trends of industrialization and urbanization are sources of contamination. Animal products with higher metal levels are a serious health risk for human. The blood, serum and edible offal (liver & kidney) were collected from sheep which were grazing in a sewerage drain polluted area along with a control group. The hematology and biochemical parameters were measured to denote the effects of Pb on sheep. The lead concentration in soil, forage and sewerage water was ranging from 06.91-15.80 mg/kg, 1.61-4.65 mg/kg and 0.01-0.15 mg/L respectively. It was calculated that the 92.22% sheep showing serum lead concentration above the recommended safe limit of 0.35 mg/L was due to uptake of Pb from environment. The RBC count, Hb and PCV showed inverse correlation with Pb concentration in serum of sheep. The effect of heavy metal on liver was measured in terms of ALT, AST & ALP and that on kidney by serum urea and creatinine. It was concluded that the biochemical tests of liver and kidney showed no effect of Pb and hence, the animals possessed the higher tolerance against Pb. The study correlates the concentration of a heavy metal (Pb) in natural environment and its dissemination to animal along with the cumulative effect on edible offals (liver and kidney) and blood parameters. The findings of this study suggested that the water, forage and soil served as continuous source of lead accumulation in sheep which may pose serious health hazards for the consumers.

Biography:

Muhammad Fiaz Qamar completed his DVM, M.Sc (Hons), and PhD from University of Veterinary & Animal Sciences Lahore-Pakistan. He is a renowned Parasitologist with more than 25 years of versatile experiences in academics including Islamia University of Bahawalpur, Govt. College University, Lahore, UVAS as well as Govt. and Private Livestock and Poultry Sector of Pakistan and abroad. He is currently a Professor in the Department of Pathobiology, College of Veterinary & Animal Sciences, Jhang. His laboratories are responsible for the isolation and identification of various pathogens including, Bacteria, Viruses, Fungus, Protozoa, Helminths, and Arthropods. In our Laboratories we also focus on Pathogenesis, Prevention/control and treatment of various Poultry and Livestock Diseases prevailing in Central Punjab. Areas of interest include developing linkages between academia and industry to get rid of emerging and re-emerging pathogens in the context of One Health including disease pathogenesis, rapid diagnosis, and prevention and control of these maladies. Dr. Qamar is highly interested in mentoring graduate students and younger faculty in the area of Communication and Leadership. He is always eager to accept challenging Jobs and gets the measurable results/outputs within shortest possible time. Initiation of Novel ideas and their implementation in veterinary Education distinguishes him from his Colleagues.

Abstract:

The prevalence of fungus was studied in relation to different months, among different species of fish, infected organs, species of fungus itself and different sampling sites. A total of five fresh water farms were randomly selected from Lahore and its surroundings; 80 fishes were sampled from each farm. These fishes were carefully examined externally with the help of a magnifying glass. The fungal infected scales and other parts were removed with the help of a scalpel and inoculated on Sabouraud Dextrose Agar (SAD). The colonies of fungus were identified on the basis of physical characters of colony, their color, shape, growth pattern, structure of sexual organs, spores size, their arrangement and septate wall. The identified species of fungus were; Aspergillus spp. (49.25%), Fusarium spp. (28.3%), Mucor spp. (8.9%), Penicillium spp (23.8%), Alternaria spp. (31.3%) and Rhizopus spp. (11.9%). The prevalence of fungal infection in different species of fishes was Hypophaimichthys molitrix (21%), Labeo rohita (19%), Cirrhinus mirrigala (16%) and Channa marulius (11%). The most infected site was caudal fin (25%) head (16.41%), abdomen (10.44%), gills (8.95%), and eyes (3.125%) in the descending order. Month wise prevalence of fungal infection had also been observed. The highest infection (25.67%) was observed in July, 2012. The prevalence percentage was in following order: June[KC1]  (18.1%), April (25%), May (23.08%), March (15.3%) and February (9.72%). The fungal infection in different sampling sites was also estimated. Farm A was proved to be highly infected with a percentage of 25 whereas Farm C appeared to have least number of infected fishes (8.75%). However, no significant difference was calculated when Chi square test was applied (P>0.05). This study showed that most of fungi isolated were considered as the natural mycoflora of fish. Most of the fungi isolated were also responsible for the production of aflatoxins. The difference of fungal infections might be linked to environmental factors (Temp, pH, and Organic matter), maintenance and cleanliness of fresh water bodies.

Biography:

Muhammad Abdullah Malik is a student of PhD at University of Agriculture Faisalabad, Punjab, Pakistan.He has completed his graduation in Doctor of Veterinary Medicine from the same Institute. The author has completed his M.Phil. in Parasitology and doing Ph.D. in Parasitology in the same Institute. The author has pubished 2 extension articles and 4 abstracts in international conferences.

Abstract:

Gastrointestinal (GI) parasitism is one of the main constraints limiting the production of livestock population. Different anthelmintics have been used by veterinary practitioners and farmers; however, unable to get rid of GI parasitism due to multiple factors including resistance against anthelmintic drugs. This study was conducted to estimate the comparative efficacy of oral formulations of levamisole (LEV) and ivermectin (IVM) against H. contortus under in vitro conditions. A total of 384 abomasa were purchased for this purpose. The lethal concentration (LC) 90 was calculated using egg hatch test (EHT), larval development test (LDT) and adult motility test (AMT). Probit transformations were performed to transform a typical sigmoid dose-response curve to linear function. A total of 100 eggs/well were incubated in different concentrations of LEV (0.172, 0.086, 0.043, 0.0215, 0.01075 and 0.00538 μl/ml) and IVM (0.0020, 0.001, 0.0005, 0.00025, 0.000125 and 0.0000625 μl/ml) was used to determine the efficacy of these drugs against eggs, larvae and adult H. contortus. The LC90 values of both the drugs were found higher than those recommended by WAAVP (0.1µg/ml) which indicated development of anthelmintic resistance (AR) against eggs and adults of H. contortus. The dose dependent response of LEV and IVM against the adult H. contortus also confirmed the resistant worms in the population. Comparative probit analysis of the two drugs indicated significantly higher (P<0.05) efficacy of IVM than LEV. The study provided useful data on the development of AR for recommending an appropriate drug for preventive and therapeutic management of helminthiosis in small ruminant livestock.

Biography:

Gurdeep kour has completed her Bachelors in 2007 at the age of 27 years from Jabalpur Veterinary College and Masters from Rajasthan Agricultural University.Had an experience working in Medical School,Ethiopia. She has published more than 8 papers in reputed journals and has been  serving  as Assistant Professor in Fiji National University.

Abstract:

The research was conducted to study the ambient temperature associated variation in serum enzymes viz. Gamma-glutamayl transferase (GGT) and Ornithine carbamoyl transferase (OCT) of hepatic function in 540 apparently healthy Marwari goats. Each ambient temperature was divided in moderate, hot and cold period. 180 blood samples in each ambient temperature period were collected. Animals were grouped according to sex (90 male and 90 female) in each ambient temperature period. Animals were further divided into age groups (5-10 months, 1-2 years and 2.5-4 years) in each ambient temperature period. 60 blood samples (30 male and 30 female) were collected in each age group. The overall mean value of OCT and  GGT were 15.4±0.30 U/l (5-35U/l) and 32.56±1.20 U/l (15-59 U/l) respectively. The mean values of OCT and GGT were significantly  (p = 0.05) higher during hot than respective moderate ambient temperature mean value. The mean   values of OCT and GGT were significantly (p =0.05) lower during cold   than respective moderate ambient temperature mean value. The sex effect was significant (p = 0.05) on the activities of serum OCT and GGT, the mean values being higher in male animals than female animals. The age effect was also significant (p = 0.05) for the activities of serum OCT and GGT, but the mean values were highest in animals of 2.5-4 Years of age. The present study may be the first to establish comprehensive values of certain serum enzymes viz. OCT and GGT of hepatic functions in Marwari breed of goat.

Biography:

Mahvish Maqbool is currently a Ph.D. student in University of Agriculture Faisalabad, Pakistan. She has completed her M.Phil. from University of Agriculture Faisalabad, Pakistan. She has worked on ticks during her M.Phil. and currently she is working on metagenomics of ticks.

Abstract:

Ticks (Acari: Ixodidae); bloodsucking parasites of domestic animals, have significant importance in transmission of diseases and causing huge economic losses. The aim of this study was to screen endophilic ticks for the Piroplasms using polymerase chain reaction in three districts Sialkot, Gujranwala and Gujarat of Punjab, Pakistan. Ticks were dissected under stereomicroscope and internal organs (midguts& salivary glands) were procured to generate pools of optimum weights. DNA extraction was done through standard protocol followed by primer specific PCR for Piroplasma spp. A total of 22.95% tick pools were found positive for piroplasma spp. In districts Sialkot and Gujranwala Piroplasma prevalence is higher in riverine animals while in Gujarat Prevalence is higher in non-riverine animals. Female animals were found more prone to piroplasma as compared to males. This study will provide useful data on the distribution of Piroplasma in the vector population of the study area and devise future recommendations for better management of ruminants to avoid subclinical and clinical infections and vector transmitted diseases.

Biography:

Mansour Mayahi has completed his DVM, 1983, Shahid Chamran University of Ahwaz, MVSc, 1992, Indian Veterinary Research Institute Izatnagar, U.P., India. and PhD, 1998. Tehran University, Tehran, Iran.. He is the dean of Faculty of Veterinary Medicine of Ahvaz. He has published more than 45 papers in reputed journals and published 9 Scientific books and has been serving as an editorial board member of reputed Iranian journal. Dr. Mayahi research  mostly  are avian diseases.

Abstract:

Micronemes are the smallest secretory organelles of the invasive stages of apicomplexan parasites and contain proteins that are important for parasite motility and host cell invasion. Molecular identification and immunogenic potency of these proteins can be used for developing of recombinant vaccine. In this study, after isolation of Eimeria necatrix from Khuzestan province, a pair of primers was designed based on the published nucleotide sequence of micronem5 gene of Eimeria necatrix LZ strain. partial sequence of a cDNA encoding 758 bp fragment of microneme 5 protein (EnMIC5) was amplified by semi- Nested RT-PCR, cloned and expressed in a Maltose Binding protein (MBP) containing expression vector (pMAL-c2x) in E. coli TG1 strain. The amplified fragment contains an open reading frame of 252 amino acids with high degree of conservation with adhesive plasma pre-kallikrein and seven hydrophilic motifs. The results of SDS-PAGE revealed that the fusion protein with molecular weight about 70 kDa was over-expressed after induction of IPTG. Western-blot results demonstrated that the expressed recombinant protein was reacted with sera of infected chicks with Eimeria necatrix, suggesting that this protein should have good immunogenicity and can be used for further studies.

Biography:

Abstract:

A study was focused on Versican (VCAN) and Gram domain containing 3 (GRAMD3) gene that are believed to be in associated with black shank colours on the domestic chickens of local breed (Jianghan fowl) in Wuhan, China. Two genes are found on the long (q) arm of chromosome 5 in specific position 14.3 and 23.2 bases on sex linked Z chromosomes according to NCBI.In this study, DNA sequencing methods were used to detect SNP of Versican and GRAMD3 genes respectively from 52 male and female chickens. Twelve (12) mutation sites were detected, seven (7) from VCAN and five (5) from GRAMD3 gene. Genotyping were done for intron 3 (T 161 C), intron 4(G 127 C), intron 5 (C 143 T), exon 7(G 603 A), exons 8( G 290 A), exon 14(G 235 C), exon 15(C 154 T) in VCAN gene and in GRAMD3, exon 2(G 199 T), intron 4 (C 81 G), exon 6(G 111 C), intron 9(C 128 T) and intron 11( A 84 G).The polymorphism was executed by polymerase chain reaction technique and genotyping (RFLP)  were done with  the data being analyzed by Z- test procedure then results compared against P≤ 0.05 with Social Science Statistic package. Among these two genes, we identified that at non-coding intron 4 of GRAMD3 gene, a significant of PË‚ 0.028717 by one-tailed results. This mutation are regarded as silent since they do not alter the amino acid composition of protein it encoded  in the entire genetic code; however it is postulated that it would be a linked marker or it would affect the shank colour through other approaches.

Biography:

Kalthoum Sana: Veterinary sanitary, regional inspector and responsible of the sub direction of Surveillance and evaluation in National Center of Zoosanitary Vigilance (38, Avenue Charles Nicolle. 1082 Cité Mahrajène-Tunis) Born in Gafsa, Tunisia on 23  december 1980. Graduated: From National School of Veterinary Medecine of Sidi Thabet on Jun 2005 (Certificate of Doctorate in Veterinary Medicine). From Veterinary national school of Afort on January 2016. France (Animal Epidemiology Diploma)

Abstract:

Foot and mouth disease (FMD) is a severe viral disease that affect many species. It causes considerable economic losses (mortality, trade restrictions ...). In 2014, Tunisian veterinary services have reported more than 140 outbreaks of FMD serotype O in cattle and sheep in several regions. It is the first report of FMD since 1999 in Tunisia. According to the World Reference Laboratory for FMD, strain present in Tunisia was closely related to that identified in Libya in 2013.  Risk identification approaches can help veterinary services to develop surveillance strategies, particularly for highly transmissible disease like FMD. In this context, this study was conducted in Tunisia to identify FMD risk areas using GIS. Risk of introduction was evaluated separately from the risk of dissemination. To evaluate risk introduction of FMD, status of neighboring countries, control measures, legal or illegal movement ware evaluated by a group of experts. For the exposure, several risk factors such as animal movement at national level and borders, animal density, accessibility were combined in order to categorize regions in four level of risks. The two maps (introduction and exposure) were subsequently combined, resulting in the risk of FMD occurrence. The validity of this result was assessed by matching very high and high-risk areas identified against FMD outbreaks reported during the last three years. Results indicate that there is perfect spatial agreement between the class map and the historical FMD outbreaks (70% of outbreaks). The main advantage in using risk mapping is to facilitate control disease, especially in countries with limited budget and focus surveillance on high-risk areas. 

Biography:

Muhammad Ijaz has completed his PhD at the age of 28 years from University of Veterinary & Animal Sciences- Lahore, Pakistan and postdoctoral studies from Cornell University Department of Population Medicine and Diagnostic Sciences. Currently, he is working as Assistant professor in the Department of Clinical Medicine & Surgery, University of Veterinary & Animal Sciences- Lahore, Pakistan. He has published more than 70 papers in reputed journals and has been serving as an editorial board member of repute.

Abstract:

Anaplasmosis is endemic in Pakistan, and is economically one of the most important bovine diseases in Asia. It reduces livestock productivity and leads to high mortality especially in young animals. This study was aimed at identifying the potential risk factors responsible for the occurrence and spread of anaplasmosis in bovine. A total of 900 (cattle = 479, buffalo = 421) blood samples were collected irrespective of age and sex from 3 distinct temporal zones of Khyber Pakhtunkhhwa (KPK) province of Pakistan. Polymerase chain reaction (PCR) technique was used for the molecular detection of anaplasmosis in comparison with microscopy of stained blood smears to compare test sensitivity for its diagnosis. Data collected on a piloted questionnaire including 11 predicting variables which were analyzed using R-statistical software, and association between the dependent and independent variables was assessed using univaraible analysis. Automated and manual approaches were exercised, producing comparable models. Key risk factors identified in all the approaches included specie of the animal, breed of animal, sex of animal, tick infestation status, previous tick history, tick control status, and acaricides use (Odds ratio >1). Also, 165 samples produced the 611bp DNA fragment specific for 16SrRNA gene of Anaplasma spp. The samples were confirmed for anaplasmosis through sequencing and BLAST queries. The findings of the current study are in line with the results of previous studies conducted in other countries identifying similar potential risk factors for anaplasmosis spread in bovine. Enhancing the protective measures for controlling the identified risk factors can reduce the spread of anaplasmosis in Pakistan.

Biography:

Moharrery has completed his PhD at the age of 35 years from University of Agricultural Sciences and he has found two sabbatical opportunities in Aarhus University from Denmark during 2007 and 2011. He has published 10 books in native language (Persian) and more than 50 papers in reputed journals and he has served as a peer reviewer for a number of academic journals and conferences.

Abstract:

In modern intensive dairy production systems, several conditions may indirectly cause increased occurrence of rumen acidosis. Production of lactic acid occurs in the rumen from fermentation of a large amount of readily-fermentable carbohydrates often results in lactic acidosis. Lactate in adapted rumen can be metabolized to propionate.  A constant concentration of lactic acid in a healthy rumen may reflect a rate of utilization approximately equal to that of production. To this end, the present study investigated the feeding of different levels of undigested NDF and starch in transition cows and their effects on lactic acid consumption in the rumen during early lactation. Thirty pregnant Holstein cows were divided into two groups, with one group being fed with wheat straw and the second group with sugar beet pulp as a source of fiber. Upon parturition, each group was divided into two sub-groups; one fed with same fiber source and the other fed with nadir fiber source. Feed intake was recorded before and after parturition. On days 10 after parturition, 10 ml rumen fluid was taken by ruminocenthesis and the ability of rumen fluid for lactic acid consumption determined. The correlation between dry matter intake (DMI) before and after parturition was analyzed using the Bayesian approach, after correction for fixed effect (fiber source treatments). There was no significant difference between treatments for lactic acid consumption. However, the highest probability density of 95% of correlation between above parameters ranged from 0.18 to 0.83 which does not contain the zero value, thus indicating a significant correlation (ó †°ρ=0.53).

Biography:

Naeem Erfani Majd is professor at the Department of Basic sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran. He has completed his PhD in 1989. He has published more than 45 papers.

Abstract:

Androgens are required to maintain the integrity of the prostate and the survival of androgen dependent epithelial cells within the gland. Anti-androgens are the primary treatment strategy for non-localized prostate cancer, but ultimately fail over time with the development of androgen independent tumors. Estrogens affect the growth and development of the prostate and may affect the development of prostate cancer. Because of the side effects of estrogen treatment alternative therapies include the use of phytoestrogens as chemo-preventative and chemotherapeutic treatment modalities.   A number of phytoestrogens have been shown to have anti-androgenic effects and anti-oxidant activities. Sesame seeds contain phytoestrogens and antioxidant agents which can affect the prostate. As a regard, this study was taken to evaluate the effect of sesame seed on the histomorphometry of prostate gland in young and aged rats. For this purpose, 20 young and 20 aged rats were selected and each one was divided into 2 groups. Control groups, which feed by pellets (28 grams) for 2 periods of 30 and 60 days. Treatment groups, which feed by 8.5-gram sesame seed and 19.5-gram food pellets for 30 days and 60 days. At the end of experiment, ventral lobe of the prostate glands were removed. The 5-6 µm sections were made using paraffin embedding technique and stained by H&E. Levels of blood testosterone were measured also. The results showed that the epithelium thickness of secretory units were increased significantly in ventral lobe of young rats prostate which feed by sesame seed. The secretory units number and diameter of the old rats were decreased significantly, and they were crushed and collapsed with numerous fold in lumen. The number, thickness and folds of secretory units were decreased significantly. The secretory cells were seen as squamous with elongated and heterochromatin nucleus. The testosterone level was increased only in young rat which feed by sesame for 60 days. Calculation: The sesame seed intense the prostate regression in old rat.