Clement Pius
National Agriculture Quarantine & Inspection Authority, New Guinea
Title: Polymorphisms and Associations of Vcan and Gramd3 Gene with Black Shank Pigmentation in Chicken
Biography
Biography: Clement Pius
Abstract
A study was focused on Versican (VCAN) and Gram domain containing 3 (GRAMD3) gene that are believed to be in associated with black shank colours on the domestic chickens of local breed (Jianghan fowl) in Wuhan, China. Two genes are found on the long (q) arm of chromosome 5 in specific position 14.3 and 23.2 bases on sex linked Z chromosomes according to NCBI.In this study, DNA sequencing methods were used to detect SNP of Versican and GRAMD3 genes respectively from 52 male and female chickens. Twelve (12) mutation sites were detected, seven (7) from VCAN and five (5) from GRAMD3 gene. Genotyping were done for intron 3 (T 161 C), intron 4(G 127 C), intron 5 (C 143 T), exon 7(G 603 A), exons 8( G 290 A), exon 14(G 235 C), exon 15(C 154 T) in VCAN gene and in GRAMD3, exon 2(G 199 T), intron 4 (C 81 G), exon 6(G 111 C), intron 9(C 128 T) and intron 11( A 84 G).The polymorphism was executed by polymerase chain reaction technique and genotyping (RFLP) were done with the data being analyzed by Z- test procedure then results compared against P≤ 0.05 with Social Science Statistic package. Among these two genes, we identified that at non-coding intron 4 of GRAMD3 gene, a significant of PË‚ 0.028717 by one-tailed results. This mutation are regarded as silent since they do not alter the amino acid composition of protein it encoded in the entire genetic code; however it is postulated that it would be a linked marker or it would affect the shank colour through other approaches.